康巧珍，郑州大学生命科学学院执行院长，博士，二级教授，博士研究生导师，郑州大学教学名师，河南省科技创新杰出人才获得者，河南省生物化学与分子生物学学会副理事长，中国实验动物学会会员。近年来先后主持完成“十二五”新药创制科技重大专项子课题1项，主持完成国家自然科学基金面上项目3项，主持完成省级教研课题1项。近年来在BLOOD等学术期刊发表学术论文40余篇，获得河南省科技进步二等奖1项；河南省高等教育教学成果二等奖1项；主编教材2部。

研究领域：分子生物学

研究方向：膜骨架蛋白与免疫调节

科研项目：

1．EPB41表达缺失对小鼠骨髓造血红系发育的影响及分子机制（81870093）

国家自然科学基金面上项目2019-2022 主持

2. 蛋白4.1R对B细胞活化及BCR介导的信号转导调控机制（81571526）

国家自然科学基金面上项目2016-2019 主持

3. Th1/Th2免疫调节剂的筛选与鉴定（174200510025）

    河南省科技创新杰出人才项目 2017-2018 主持

4. 蛋白4.1R对肥大细胞脱颗粒调控的分子机制（81373119）

    国家自然科学基金面上项目2014-2017 主持

5. 候选Th1免疫增强新药rMBP-NAP抗肿瘤研究（2012ZX09103-301-022）

新药创制国家科技重大专项子课题2012-2015 主持

6. 4.1R对CD4+T细胞活化及TCR/CD3介导的信号转导调控机制（30972707）

国家自然科学基金2010-2012 主持

教研项目：

1. 生物学研究生课程体系建设与优化研究（2017SJGLX020Y）

河南省研究生教育教学改革研究与实践项目2018-2019 主持

2. 研究生课程建设试点 (YJSKCSD201502)

郑州大学研究生教改项目 2015-2017 主持

3. 生物工程研究生实践教学改革（YJSJY201702）

郑州大学研究生实践教学改革项目2017-2019 主持

代表论文：

1. Chen L, Wang T, Ji X, Ding C, Liang T, Liu X, Lu J, Guo X, Kang Q*, Ji Z. Cytoskeleton protein 4.1R suppresses murine keratinocyte cell hyperproliferation via activating the Akt/ERK pathway in an EGFR-dependent manner. Exp Cell Res. 2019 ;15;384(2):111648.

2. Wang C, Li K, Men Y, Ding C, Du J, Liang T, Ji Z, Chen L, Wang T, Kang Q*. Protein 4.1B Suppresses Tumor Metastasis by Regulating Epithelial-mesenchymal Transition Progression in Melanoma Cells. Int J Med Sci. 2019;16(4):529-536

3. Lu J, Cui L, Lin S, Hao L, Cao N, Yi J, Liu X, Lu L, Kang Q*. Short communication: Global transcriptome analysis of Lactococcus lactis ssp. lactis in response to gradient freezing. J Dairy Sci. 2019;102(5):3933-3938

4. Wang P, Ke Z, Yi J, Liu X, Hao L, Kang Q*, Lu J .Effects of β-cyclodextrin on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp.99-125, Industrial Crops & Products. 2019，129： 688-693

5.Kang Q, Zhang X, Cao N, Chen C, Yi J, Hao L, Ji Y, Liu X, Lu J. EGCG enhances cancer cells sensitivity under 60Coγ radiation based on miR-34a/Sirt1/p53. Food Chem Toxicol. 2019;133:110807

6.Kang Q, Chen S, Li S, Wang B, Liu X, Hao L, Lu J. Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction. Int J Biol Macromol. 2019,124:1137-1144.

7. Fan D, Li J, Li Y, Guo Y, Zhang X, Wang W, Liu X, Liu J, Dai L, Zhang L, Kang Q, Ji Z. Protein 4.1R negatively regulates CD8⁺ T-cell activation by modulating phosphorylation of linker for activation of T cells. Immunology. 2019;157(4):312-321

8. Lin S, Kang Q, Pan D, Liu X, Lu L, Hao L and Lu J. Identification of lyoprotectants released from gradient-freezing pretreated entocyte in Lactococcus lactis subsp. lactis IL1403 using comparative proteomics method. Current Topics in Nutraceutical Research. 2019, 17(2): 122-134.

9. Qu X, Zhang S, Wang S, Wang Y, Li W, Huang Y, Zhao H, Wu X, An C, Guo X, Hale J, Li J, Hillyer CD, Mohandas N, Liu J, Yazdanbakhsh K, Vinchi F, Chen L, Kang Q*, An X. TET2 deficiency leads to stem cell factor-dependent clonal expansion of dysfunctional erythroid progenitors. Blood. 2018;132(22):2406-2417

10. Wang T, Du M, Ji Z, Ding C, Wang C, Men Y, Liu S, Liang T, Liu X, Kang Q*. Recombinant protein rMBP-NAP restricts tumor progression by triggering antitumor immunity in mouse metastatic lung cancer.Can J PhysiolPharmacol, 2018, 96(2):113-119.

11. Ding C, Li L, Zhang Y, Ji Z, Zhang C, Liang T, Guo X, Liu X, Kang Q*. Toll-like receptor agonist rMBP-NAP enhances antitumor cytokines production and CTL activity of peripheral blood mononuclear cells from patients with lung cancer. Oncol Lett. 2018 ;16(4):4707-4712

12. Wang Y, Zhang H, Kang Q, Liu J, Weng H, Li W, Mohandas N, An X, Chen L. Protein 4.1N is required for the formation of the lateral membrane domain in human bronchial epithelial cells. Biochim Biophys Acta Biomembr. 2018;1860(5):1143-1151.

13. Cui Y, Liu X, Li S, Hao L, Du J, Gao D, Kang Q, Lu J. Extraction, characterization and biological activity of sulfated polysaccharides from seaweed Dictyopteris divaricate.Int J Biol Macromol, 2018， 117:256-263.

14. Pan D, Zhang D, Hao L, Lin S, Kang Q, Liu X, Lu L, Lu J. Protective effects of soybean protein and egg white protein on the antibacterial activity of nisin in the presence of trypsin.Food Chem, 2018, 239:196-200.

15. Ning S, Kang Q, Fan D, Liu J, Xue C, Zhang X, Ding C, Zhang J, Peng Q, Ji Z.Protein 4.1R is Involved in the Transport of 5-Aminolevulinic Acid by Interaction with GATs in MEF Cells. PhotochemPhotobiol. 2018，94(1):173-178.

16. Lu J, Wang P, Ke Z, Liu X, Kang Q, Hao L.Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp. 99-125.Int J Biol Macromol. 2018, 114:922-928.15.

17. Wang Y, Zhang H, Kang Q, Liu J, Weng H, Li W, Mohandas N, An X, Chen L. Protein 4.1N is required for the formation of the lateral membrane domain in human bronchial epithelial cells. BiochimBiophys Acta Biomembr. 2018，1860(5):1143-1151.

18. Yan H, Wang Y, Qu X, Li J, Hale J, Huang Y, An C, Papoin J, Guo X, Chen L, Kang Q, Li W, Schulz VP, Gallagher PG, Hillyer CD, Mohandas N, An X. Distinct roles for TET family proteins in regulating human erythropoiesis. Blood. 2017;129(14):2002-2012.

19. Li S, Hao L, Kang Q, Cui Y, Jiang H, Liu X, Lu J. Purification, characterization and biological activities of a polysaccharide from Lepidium meyenii leaves.Int J Biol Macromol. 2017, 103:1302-1310.

20. Liu X, Fu G, Ji Z, Huang X, Ding C, Jiang H, Wang X, Du M, Wang T, Kang Q*.A Recombinant DNA Plasmid Encoding the sIL-4R-NAP Fusion Protein Suppress Airway Inflammation in an OVA-Induced Mouse Model of Asthma.Inflammation. 2016;39(4):1434-40.

21.Chen L, Wang T, Wang Y, Zhang J, Qi Y, Weng H, Kang Q, Guo X, Baines AJ, Mohandas N, An X. Protein 4.1G Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryonic Fibroblasts through the β1 Integrin Pathway. J Biol Chem. 2016;291(5):2170-80

22. Wang T, Liu X, Ji Z, Men Y, Du M, Ding C, Wu Y, Liu X, Kang Q*. Antitumor and immunomodulatory effects of recombinant fusion protein rMBP-NAP through TLR-2 dependent mechanism in tumor bearing mice. Int Immunopharmacol. 2015;29(2):876-883.

23. Lu, J, Song, Q, Ji, Z, Liu, X, Wang, T, Kang Q*. Fermentation optimization of maltose-binding protein fused to neutrophil-activating protein from Escherichia coli TB1. Electronic Journal of Biotechnology. 2015, 18(4)：281-285.

24. Liu X, Zhou Q, Ji Z, Fu G, Li Y, Zhang X, Shi X, Wang T, Kang Q*.

Protein 4.1R attenuates autoreactivity in experimental autoimmune encephalomyelitis by suppressing CD4(+) T cell activation.Cell Immunol. 2014;292(1-2):19-24.

25. Wang H, Chen X, Chen Y, Sun L, Li G, Zhai M, Zhai W, Kang Q, Gao Y, Qi Y.Antitumor activity of novel chimeric peptides derived from cyclinD/CDK4 and the protein transduction domain 4.Amino Acids. 2013;44(2):499-510.

26. Ji Z, Shi X, Liu X, Shi Y, Zhou Q, Liu X, Li L, Ji X, Gao Y, Qi Y, Kang Q*.The membrane-cytoskeletal protein 4.1N is involved in the process of cell adhesion, migration and invasion of breast cancer cells.

Exp Ther Med. 2012 ;4(4):736-740.

27. Wu D, Gao Y, Chen L, Qi Y, Kang Q, Wang H, Zhu L, Ye Y, Zhai M.Anti-tumor effects of a novel chimeric peptide on S180 and H22 xenografts bearing nude mice .Peptides. 2010;31(5):850-64.

28. Kang Q, Wang T, Zhang H, Mohandas N, An X.A Golgi-associated protein 4.1B variant is required for assimilation of proteins in the membrane . J Cell Sci. 2009;122(Pt 8):1091-9.

29. Kang Q, Yu Y, Pei X, Hughes R, Heck S, Zhang X, Guo X, Halverson G, Mohandas N, An X.Cytoskeletal protein 4.1R negatively regulates T-cell activation by inhibiting the phosphorylation of LAT. Blood. 2009;113(24):6128-37.

30. 林松洋,郝利民,刘鑫,鲁来政,康巧珍,鲁吉珂.乳酸菌耐盐分子机制研究进展[J].食品科学,2018,39(03):295-301.

31. 张成龙,康巧珍,汲振余,郭勋,杜娟,达甜甜,汪文,刘鑫*.VISTA-Ig融合蛋白的表达及其包涵体复性研究 [J].生物技术,2018,28(01):30-35.

32. 江慧,康巧珍,黄夏冰,张成龙,刘鑫*.小鼠PD-1胞外段重组蛋白表达及其包涵体复性 [J].郑州大学学报(理学版),2017,49(02):112-116.

33. 门颖丽,康巧珍,王小龙,江慧,刘诗梦,汲振余,王婷,刘鑫*.兔抗鼠IL-12p35多克隆抗体的制备及应用 [J].南昌大学学报(理科版),2017,41(01):72-76.

34. 鲁吉珂,黄进勇,王时征,翁海波,陈鲤翔,位芳,刘鑫,郭亚楠,王婷,楚德强,韩绍印,康巧珍.“现代生物学实验技术”在线开放课程的探索与实践 [J].高校生物学教学研究(电子版),2017,7(02):9-12.

35. 门颖丽, 康巧珍,丁聪,刘诗梦,江慧,王晓东,汲振余,刘鑫,王婷.蛋白4.1家族在小鼠黑色素瘤细胞中的表达及其对细胞增殖的影响 [J].南方医科大学学报,2016,36(05):649-654.

36. 王晓东,康巧珍,王小龙,李树芳,王婷,刘鑫,汲振余.HP-NAP对肝癌H22荷瘤小鼠肿瘤生长的抑制作用 [J].郑州大学学报(医学版),2016,51(05):595-598.

37. 门颖丽,康巧珍,王小龙,杜明宣,黄夏冰,张亚萌,汲振余,王婷,刘鑫.MBP在小鼠肝癌H22荷瘤模型中的抗肿瘤作用 [J].郑州大学学报(医学版),2016,51(03):313-316.

38. 孙博强,康巧珍,潘周浩,周苗,杨敏,刘鑫. DNCB诱导昆明小鼠特应性皮炎模型的建立 [J].生物技术世界,2016(05):12-13.

39. 门颖丽,王小龙,丁聪,王晓东,汲振余,王婷,康巧珍,刘鑫*.兔抗小鼠IL-23p19多克隆抗体的制备和鉴定 [J].细胞与分子免疫学杂志,2016,32(05):688-692.

40. 黄夏冰,康巧珍,傅国,汲振余,刘鑫.GST-NAP融合蛋白可溶性表达及柱上切割GST标签 [J].郑州大学学报(理学版),2015,47(04):94-98.

41. 黄夏冰,康巧珍,王晓东,丁聪,江慧,刘诗梦,刘鑫.实验性自身免疫性脑脊髓炎(EAE)C57BL/6J小鼠模型构建 [J].河南医学研究,2015,24(08):8-10.

42. 王小龙,康巧珍,门颖丽,黄夏冰,张亚萌,刘鑫.rMBP-NAP依赖IL-12/IL-23轴对肝癌H22荷瘤小鼠的抑瘤作用研究 [J].河南医学研究,2015,24(08):5-8.

43.范丹丹李懿李建辉宁姝威禹丽娜张立果范天黎杨小静裘一兵，康巧珍， 汲振余. 细胞膜骨架蛋白4.1R对光动力效应的影响[J] 中华医学杂志2014,94(48):

教材与专著:

1. 《现代生物学实验技术数字课程》 主编 2017高教出版社

出版社书号: ISBN:978-7-89510-079-4

2. 《分子生物学》主编 2015 郑州大学出版社 书号：ISBN978-7-5645-2189-9

3. 《生物化学实验原理与技术》编委 2010化学工业出版社“十二五”规划教材

4. 《生命科学导论》编委 2004 郑州大学出版社 ISBN7-81048-932-1

5. 《实验动物与动物实验技术》副主编 2003 中国中医药出版社
6. 《实验动物学》教材 1991广东高等教育出版社 参编

教学科研奖：

1. 《生物学研究生创新课题体系建设与实践》2019年获河南省高等教育教学成果（研究生教育）二等奖。

2. 《蛋白4.1R对T细胞活化的机制与应用研究》2015年获河南省科学技术进步二等奖。