基本信息
李占先，教授，硕士生导师
联系方式：15093275445
邮箱：lizx@zzu.edu.cn
实验室：化学实验楼121
通讯地址：郑州大学化学学院450001
主持国家自然基金两项，参与三项；主持教育部新教师联合基金一项，以第一申请人授权发明专利7项，在Biosens. Bioelectron.、J. Phys. Chem. C、Org. Lett.、Chem. Commun.、Inorg. Chem.、Langmuir、Anal. Chem.、Sens. Actuat. B、J. Mater. Chem. C等杂志发表SCI论文50多篇，单篇最高引用次数257次（至2020年10月）。
教育背景
2001.09-2006.05中科院理化所，博士学位
1997.09-2001.07郑州大学化学系，本科学位
工作履历
2019.04-郑州大学化学学院，教授
2016.01-2019. 04郑州大学化学学院，直聘教授
20014.01-2015.12郑州大学化学学院，副教授
2008.10-2013.12郑州大学化学学院，讲师
2006.07-2008.06北京大学，稀土材料化学及应用国家重点实验室，博士后
2017.8-2018.8，美国新泽西理工学院，国家留学基金委访问学者
研究领域
有机分子荧光材料、稀土上转换发光材料、碳点的设计、合成及其在生物监测方面的应用
主持科研项目情况：
1、国家自然科学基金青年基金项目，21601158、精氨酸的能量转移型复合纳米近红外比率荧光探针及其生物成像、2017/01-2019/12、19万元、结题、主持。
2、国家自然科学基金联合基金项目，U1504203、能量转移型肼的上转换纳米复合比率荧光探针的制备及荧光成像、2016/01-2018/12、27万元、结题、主持。
3、教育部新教师联合基金，树枝状高分子的荧光离子检测研究、2014/01-2016/12、结题、主持。
4郑州大学优秀青年教师基金，碳点/有机复合肼的纳米比率荧光探针的制备及其生物成像应用研究，2017/01-2019/12、30万元、结题、主持
研究成果（Selected publications）
1. Yang, C.; Li, Yueyue.; Wu, N.; Zhang, Y.; Feng, W.; Yu, M.*;Li, Z.*, Ratiometric upconversion luminescence nanoprobes for quick sensing of Hg2+and cells imaging.Sens. Actuators B,2021,326,128841.
2. Li, Y. Y.; Ban, Y. N.; Wang, R. H.; Wang, Z.;Li, Z. X.*;Fang, C. J.; Yu, M. M.*, FRET-based Ratiometric Fluorescent Detection of Arginine in Mitochondrion with a Hybrid Nanoprobe.Chin. Chem. Lett.,2020,31, 443–446.
3. Hou, Y.; Liu, H.X.; Li, Z.X.*; Zhang, H.Y.*; Wei, L.H.; Yu, M.M.* One-step synthesis of mitochondrion-targeted fluorescent carbon dots and fluorescence detection of silver ions.Anal. Methods,2020,12, 2835–2840.
4. Bu, D.D.; Song, H.H.; Li, Z.X.*; Wei, L.H.; Zhang, H.Y.*; Yu, M.M.* Carbon-dot-based ratiometric fluorescent probe of intracellular zinc ion and persulfate ion with low dark toxicity.Luminescence,2020, 1–9. DOI: 10.1002/bio.3894.
5. Liu, W.J.; Wang, Y.Y.; Wu, N.; Feng, W.; Li, Z.X.*; Wei, L.H.; Yu, M.M.* A mitochondrion-targeting fluorescent probe for hypochlorite anion in living cells.Spectro. chim. Acta A,2020,240, 118564.
6. Li,Z. X.;Liu, H. X.; Li, H. X.;Tsou, Y.-H.;Gao, Y. L.;Xu, X. Y.*; Du, W. W.; Wei, L. H.;Yu, M. M.*, Lysosome-targeting NIR ratiometric luminecent upcoversion nanoprobe toward arginine,Sens. Actuators B,2019,280,94–101.
7. Wang, Y. Y.; Liu, H. X.; Song, H. H.; Yu, M.M.*;Wei, L. H.;Li, Z. X.*,Synthesis of dual-emission fluorescent carbon quantum dots and their ratiometric fluorescence detection for arginine in 100% water solution.New J. Chem.,2019,43, 13234–13239.
8. Song, H. H.; Du, W. W.; Liu, C. X.;Li,Z. X.*; Zhang H. Y.*; Wei, L. H.; Yu, M. M.* Self-Assembled Micellar Nanosensor toward pH with high photo-stability and its application in living cells,Sens. Actuators B,2018,273, 927–934.
9. Li, H. X.; Dong, H.;Yu, M. M.; Liu, C. X.;Li, Z. X.*;Wei, L. H.; Sun, L.-D. *; Zhang, H. Y.* NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells with Hemicyanine Derivative-Assembled Upconversion Nanophosphors,Anal. Chem.,2017,89, 8863−8869.
10. Yu, M. M.*; Du, W. W.; Li, H. X.; Zhang, H. Y.*;Li, Z. X.* Near-infrared ratiometric fluorescent detection of arginine in lysosome with a new hemicyanine derivative.Biosens. Bioelectron.,2017,92, 385–389.
11. Li, Z. X.*; Zhang, W. Y.; Liu, C. X.; Yu, M. M.*; Zhang, H. Y.*; Guo, L.; Wei, L. H. A colorimetric and ratiometric fluorescent probe for hydrazine and its application in living cells with low dark toxicity.Sens. Actuators B,2017,241, 665–671.
12. Shi, B. J.; He, Y. Y.;Zhang, P. P.; Wang, Y. L.; Yu, M. M.*;Zhang, H. Y. * Wei, L. H.; Li, Z. X.* Turn on fluorescent detection of hydrazine with a 1,8-naphthalimde derivative,Dyes Pigments, 2017, 152-159.
13. An, Z.;Li, Z. X.*; He, Y.; Shi, B.; Wei, L.; Yu, M. M.* Ratiometric luminescent detection of hydrazine with a carbon dots-hemicyanine nanohybrid system.RSC Adv.,2017,7, 10875–10880.
14. He, Y. Y.; Li, Z. X.*; Jia, Q. Y.; Shi, B. J.; Zhang, H. Y..*;Wei, L. H.; Yu, M. M..* Ratiometric fluorescent detection of acidic pH in lysosome with carbon nanodots, Chin. Chem. Lett.2017,28, 1969–1974.
15. Li, Z. X.; Li, H. X.; Shi, C. X.; Yu, M. M.; Wei, L. H.; Ni Z. H.* Nanomolar colorimetric quantitative detection of Fe3+and PPi with high selectivity.Spectro. chim. Acta A,2016,159, 249–253.
16. Yu, M. M.*, Du, W. W.; Zhou, W.; Li, H. X.; Liu, C. X.; Wei, L. H.;Li, Z. X.*; Zhang, H. Y. A 1,8-naphthalimide-based chemosensor with an off-on fluorescence and lifetime imaging response for intracellular Cr3+and further for S2−.Dyes Pigments,2016, 126, 279–285.
17. Li, Z. X.*; Li, H. X.; Shi, C. X.; Zhang, W. Y.; Zhou, W.; Wei, L. H.; Yu, M. M.* Naked-eye-based highly selective sensing of Fe3+and further for PPi with nano copolymer film.Sens. Actuators B,2016, 226, 127–134.
18. Zhang, W.; Liu, X.; Zhang, H.; Feng, C.; Liu, C.; Yu, M.*; Wei, L. andLi, Z.*A fluorescent probe for benzenethiols and its application on test paper, in water samples and living cells,J. Mater. Chem. C,2015,3, 8248--8254.
19. Liu, C. X.; Shi, C. X.; Li, H. X.; Du, W. W.;Li, Z. X.*; Wei, L. H.; Yu, M. M.* Nanomolar fluorescent quantitative detection of formaldehyde with a 8-hydroxyquinoline derivative in aqueous solution and electrospun nanofibers.Sens. Actuators B,2015,219, 185–191.
20. Li, Z. X.*; Zhang, W. Y.; Liu, X. J.; Liu, C. X.; Yu, M. M.*; Wei L. H.* Naked-eye-based selective detection of pyrophosphate with Zn2+complex in aqueous solution and electrospun nanofibers.RSC Adv.,2015,5, 25229–25235.
21. Liu, C. X.; Xu, J.; Yang, F.; Zhou, W.;Li, Z. X.*; Wei L. H.; Yu, M. M.* Nanomolar Cu2+and F–naked-eye detection with a 1,8-naphthalimide-based colorimetric probe.Sens. Actuators B,2015,212, 364–370.
22. Du, W. W.; Xu, J.; Li, H. X.; Feng, C. C.; Yu, M. M.*;Li Z. X.*; Wei L. H. Naked-eye and fluorescent detection of basic pH and F−with a 1,8-naphthalimide-based multifunctional probe.RSC Adv.,2015,5, 15077–15083.
23. Zhou, W.; Dong, H.; Yan, H.; Shi, C. X.; Yu, M. M.*; Wei, L. H.;Li, Z. X.*HCHO-reactive molecule with dual-emission-enhancement property for quantitatively detecting HCHO in near 100% water solution.Sens. Actuators B,2015,209, 664–669.
24. Liu, X. J.; Zhang, W.Y.; Li, C. X.; Zhou, W.;Li, Z. X.*; Yu, M. M.*; Wei, L. H. Nanomolar detection of Hcy, GSH and Cys in aqueous solution, test paper and living cells.RSC Adv.,2015,5, 4941–4946.
25. Li, Z. X.*; Liu, X. J.; Zhao, W. Y.; Wang, S.; Zhou, W.; Wei, L. H.; Yu, M. M.* Naked-eye detection of C1–C4 alcohols based on ground-state intramolecular proton transfer.Anal. Chem.,2014,86, 2521−2525.
授权专利：
• 李占先、宋欢欢、刘春霞、于明明、魏柳荷，一种检测细胞内pH值的聚合物纳米探针及其制备方法以及应用。专利申请日：2018.03.01，授权公告日：2020.09.04，中国，ZL201810170768.1。
• 李占先、班亚楠、于明明、杜玮玮、李海霞、魏柳荷，一种溶酶体定位荧光探针在近红外比率检测肼中的应用。2019.7，中国，ZL201610950531.6。
• 李占先、班亚楠、李海霞、于明明、魏柳荷，一种检测精氨酸的上转换发光纳米传感材料及在精氨酸检测中的应用。2019.7，中国，ZL201710141382.3。
• 李占先、班亚楠、于明明、李海霞、刘春霞、魏柳荷，一种用于制备近红外比率发光上转换纳米材料的化合物、制备方法及应用。2019.3，中国，专利号：ZL201611120950.3。
• 李占先、于明明、魏柳荷、刘兴江、赵万莹、周婉，一种低级醇检测探针、制备方法及应用。2015.4，中国，专利号：ZL201310198342.4。
• 李占先、于明明、张利锋、虞明、魏柳荷、刘金霞，一种检测细胞内锌离子的分子荧光开关及其应用。2013.4，中国，专利号：ZL201010181802.9。
• 李占先、于明明、魏柳荷、张利锋、赵万莹、王丽娜，萘联苯衍生物金属离子荧光探针的制备及其识别检测生物样品或环境中Fe3+和/或Cu2+的应用。2013.4，中国，专利号：ZL201110169892.4。
奖励与荣誉（Selected honors/awards）
研究生及本科毕业生所获部分奖励
2011年
张利锋在2010年的研究生论坛获得"最佳宣讲人"称号并获得校级优秀毕业论文
2012年
赵万莹被评为省级优秀研究生并获得校级优秀毕业论文
2014年
刘兴江获得校级及省级优秀毕业生
刘兴江获得省级优秀毕业论文
张文英获得郑州大学优秀科研奖
2015年
张文英获得国家奖学金
2016年
李海霞获得国家奖学金
张文英获得省级优秀毕业论文
2017年
李海霞获得郑州大学优秀毕业生
指导的本科生毕业论文多次获得院优秀毕业论文（虞明，王稳，刘自平等）
Curriculum vitae
Profile
Name (in pinyin and Chinese): Zhanxian Li/李占先
Business Address: College of Chemistry, No. 100 of Kexue Road, High New-Tech Development Area, Zhengzhou, Henan province, China
Work Phone: 0371-67783126
Mobile Phone: 15093275445
E-mail: lizx@zzu.edu.cn
Educational
Sep., 2001-Jun., 2006 Technical Institute of Physics and Chemistry, CAS
Ph. D, Organic Chemistry
Sep., 1997-Jun., 2001 Zhengzhou University
MS, Chemistry
Experience
Apr., 2019- Zhengzhou University
Professor
Aug., 2017-Aug., 2018 USA, New Jersey Institute of technology (NJIT),
Visiting Scholar
Jan., 2014- Apr., 2019 Zhengzhou University
Associate Professor
Oct., 2008-Nov., 2013 Zhengzhou University
Lecturer
Jul., 2006-Jun., 2008 Peking University
Post Doctor
Research area
Design and Synthesis of Organic Molecular Fluorescent Materials, Rare Earth Upconversion Luminescent Materials, Carbon Dots and Their Applications in Biomonitoring
Selected publications
1. Yang, C.; Li, Yueyue.; Wu, N.; Zhang, Y.; Feng, W.; Yu, M.*;Li, Z.*, Ratiometric upconversion luminescence nanoprobes for quick sensing of Hg2+and cells imaging.Sens. Actuators B,2021,326,128841.
2. Li, Y. Y.; Ban, Y. N.; Wang, R. H.; Wang, Z.;Li, Z. X.*;Fang, C. J.; Yu, M. M.*, FRET-based Ratiometric Fluorescent Detection of Arginine in Mitochondrion with a Hybrid Nanoprobe.Chin. Chem. Lett.,2020,31, 443–446.
3. Hou, Y.; Liu, H.X.; Li, Z.X.*; Zhang, H.Y.*; Wei, L.H.; Yu, M.M.* One-step synthesis of mitochondrion-targeted fluorescent carbon dots and fluorescence detection of silver ions.Anal. Methods,2020,12, 2835–2840.
4 Bu, D.D.; Song, H.H.; Li, Z.X.*; Wei, L.H.; Zhang, H.Y.*; Yu, M.M.* Carbon-dot-based ratiometric fluorescent probe of intracellular zinc ion and persulfate ion with low dark toxicity.Luminescence,2020, 1–9. DOI: 10.1002/bio.3894.
5 Liu, W.J.; Wang, Y.Y.; Wu, N.; Feng, W.; Li, Z.X.*; Wei, L.H.; Yu, M.M.* A mitochondrion-targeting fluorescent probe for hypochlorite anion in living cells.Spectrochim. Acta A,2020,240, 118564.
6.Li,Z. X.;Liu, H. X.; Li, H. X.;Tsou, Y.-H.;Gao, Y. L.;Xu, X. Y.*; Du, W. W.; Wei, L. H.;Yu, M. M.*, Lysosome-targeting NIR ratiometric luminescent upcoversion nanoprobe toward arginine,Sens. Actuators B,2019,280,94–101.
7. Wang, Y. Y.; Liu, H. X.; Song, H. H.; Yu, M.M.*;Wei, L. H.;Li, Z. X.*,Synthesis of dual-emission fluorescent carbon quantum dots and their ratiometric fluorescence detection for arginine in 100% water solution.New J. Chem.,2019,43, 13234–13239.
8. Song, H. H.; Du, W. W.; Liu, C. X.;Li,Z. X.*; Zhang H. Y.*; Wei, L. H.; Yu, M. M.* Self-Assembled Micellar Nanosensor toward pH with high photo-stability and its application in living cells,Sens. Actuators B,2018,273, 927–934.
9. Li, H. X.; Dong, H.;Yu, M. M.; Liu, C. X.;Li, Z. X.*;Wei, L. H.; Sun, L.-D.*; Zhang, H. Y.* NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells with Hemicyanine Derivative-Assembled Upconversion Nanophosphors,Anal. Chem.,2017,89, 8863−8869.
10. Yu, M. M.*; Du, W. W.; Li, H. X.; Zhang, H. Y.*;Li, Z. X.* Near-infrared ratiometric fluorescent detection of arginine in lysosome with a new hemicyanine derivative.Biosens. Bioelectron.,2017,92, 385–389.
11.Li, Z. X.*; Zhang, W. Y.; Liu, C. X.; Yu, M. M.*; Zhang, H. Y.*; Guo, L.; Wei, L. H. A colorimetric and ratiometric fluorescent probe for hydrazine and its application in living cells with low dark toxicity.Sens. Actuators B,2017,241, 665–671.
12. Shi, B. J.; He, Y. Y.;Zhang, P. P.; Wang, Y. L.; Yu, M. M.*;Zhang, H. Y. * Wei, L. H.; Li, Z. X.* Turn on fluorescent detection of hydrazine with a 1,8-naphthalimde derivative,Dyes Pigments, 2017, 152-159.
13. An, Z.;Li, Z. X.*; He, Y.; Shi, B.; Wei, L.; Yu, M. M.* Ratiometric luminescent detection of hydrazine with a carbon dots-hemicyanine nanohybrid system.RSC Adv.,2017,7, 10875–10880.
14. He, Y. Y.; Li, Z. X.*; Jia, Q. Y.; Shi, B. J.; Zhang, H. Y..*;Wei, L. H.; Yu, M. M..* Ratiometric fluorescent detection of acidic pH in lysosome with carbon nanodots,Chinese Chem. Lett.2017,28, 1969–1974.
15. Li, Z. X.; Li, H. X.; Shi, C. X.; Yu, M. M.; Wei, L. H.; Ni Z. H.* Nanomolar colorimetric quantitative detection of Fe3+and PPi with high selectivity.Spectro. Chim. Acta A,2016,159, 249–253.
16. Yu, M. M.*, Du, W. W.; Zhou, W.; Li, H. X.; Liu, C. X.; Wei, L. H.;Li, Z. X.*; Zhang, H. Y. A 1,8-naphthalimide-based chemosensor with an off-on fluorescence and lifetime imaging response for intracellular Cr3+and further for S2−.Dyes Pigments,2016, 126, 279–285.
17.Li, Z. X.*; Li, H. X.; Shi, C. X.; Zhang, W. Y.; Zhou, W.; Wei, L. H.; Yu, M. M.* Naked-eye-based highly selective sensing of Fe3+and further for PPi with nano copolymer film.Sens. Actuators B,2016, 226, 127–134.
18. Zhang, W.; Liu, X.; Zhang, H.; Feng, C.; Liu, C.; Yu, M.*; Wei, L. andLi, Z.*A fluorescent probe for benzenethiols and its application on test paper, in water samples and living cells,J. Mater. Chem. C,2015,3, 8248—8254.
19. Liu, C. X.; Shi, C. X.; Li, H. X.; Du, W. W.;Li, Z. X.*; Wei, L. H.; Yu, M. M.* Nanomolar fluorescent quantitative detection of formaldehyde with a 8-hydroxyquinoline derivative in aqueous solution and electrospun nanofibers.Sens. Actuators B,2015,219, 185–191.
20.Li, Z. X.*; Zhang, W. Y.; Liu, X. J.; Liu, C. X.; Yu, M. M.*; Wei L. H.* Naked-eye-based selective detection of pyrophosphate with Zn2+complex in aqueous solution and electrospun nanofibers.RSC Adv.,2015,5, 25229–25235.
21. Liu, C. X.; Xu, J.; Yang, F.; Zhou, W.;Li, Z. X.*; Wei L. H.; Yu, M. M.* Nanomolar Cu2+and F–naked-eye detection with a 1,8-naphthalimide-based colorimetric probe.Sens. Actuators B,2015,212, 364–370.
22. Du, W. W.; Xu, J.; Li, H. X.; Feng, C. C.; Yu, M. M.*;Li Z. X.*; Wei L. H. Naked-eye and fluorescent detection of basic pH and F−with a 1,8-naphthalimide-based multifunctional probe.RSC Adv.,2015,5, 15077–15083.
23. Zhou, W.; Dong, H.; Yan, H.; Shi, C. X.; Yu, M. M.*; Wei, L. H.;Li, Z. X.*HCHO-reactive molecule with dual-emission-enhancement property for quantitatively detecting HCHO in near 100% water solution.Sens. Actuators B,2015,209, 664–669.
24. Liu, X. J.; Zhang, W.Y.; Li, C. X.; Zhou, W.;Li, Z. X.*; Yu, M. M.*; Wei, L. H. Nanomolar detection of Hcy, GSH and Cys in aqueous solution, test paper and living cells.RSC Adv.,2015,5, 4941–4946.
25.Li, Z. X.*; Liu, X. J.; Zhao, W. Y.; Wang, S.; Zhou, W.; Wei, L. H.; Yu, M. M.* Naked-eye detection of C1–C4 alcohols based on ground-state intramolecular proton transfer.Anal. Chem.,2014,86, 2521−2525.
