张开翔-药学院

张开翔

10AE8

张开翔

郑州大学药学院教授，博士生导师，国家优青

联系方式：zhangkx@zzu.edu.cn

个人简介

张开翔，郑州大学药学院教授，博士生导师，国家优青。主要研究方向为：1）基于CRISPR生物探针的基因突变原位成像分析；2）基于核酸纳米材料的药物递送和基因治疗。相关研究成果以第一作者或通讯作者在Nat. Commun., Sci. Adv., J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater., Chem. Soc. Rev., 等期刊发表研究论文50余篇, 累计发表SCI收录论文100余篇，被引5000余次，作为项目负责人承担国家自然科学基金项目4项。

教育和工作经历

2023.5 – 至今郑州大学药学院教授

2017.7 – 2023.5 郑州大学药学院副教授

2012.8 – 2014.8 美国加州大学尓湾分校药学院联合培养博士

2011.9 – 2017.7 清华大学化学系博士

2007.9 – 2011.7 同济大学材料科学与工程系学士

荣誉称号及奖励

2021年国家优青

2020年河南省高校科技创新人才

2019年中原青年拔尖人才

2017年清华大学“启航奖”金奖

主持的科研项目

1. 国家自然科学基金优秀青年科学基金项目，22122409, CRISPR生物探针构建与生物传感，2022/01-2024/12，在研，主持。

2. 国家自然科学基金面上项目，22377110，基于RNA腺苷脱氨酶的核酸探针构建及体内基因突变原位成像研究，2024/01-2027/12，在研，主持。

3. 国家自然科学基金联合培育项目，U2004197，外泌体的单颗粒多参数荧光原位成像分析方法设计及应用研究，2021/01-2023/12，在研，主持

4. 国家自然科学基金青年项目，21904119，近端结合CRISPR探针构建及细胞核基因点突变直接成像研究，2020/01-2022/12，结题，主持。

5. 河南省优势学科联合培育重点项目，222301420019，基于外泌体表面蛋白单分子成像的临床食管癌检测研究，2022/01-2024/12，在研，主持

6. 中原青年拔尖人才，ZYQR201912191，数字化尿液外泌体检测芯片用于膀胱癌早期筛查及耐药性分析，2020/01-2021/12，在研，主持。

7. 河南省高校科技创新人才，21HASTIT043，单细胞、单颗粒、单分子分析，2021/01-2023/12，在研，主持。

8. 河南省科技攻关项目，基于酵母天然机制的一步化CRISPR/Cas9纳米药物制备及基因，疗研究，项目编号：192102310147，2020/01-2021/12，结题，主持。

发明专利

1. 张开翔，李亚楠，裴怡然，徐汝，高华，史进进，张振中；基于CRISPR/Cas13a系统的一步法无靶标扩增可视化 RNA病毒检测方法，2022.12.13，中国专利，申请，CN202211596224.4

2. 张开翔，李亚楠，裴怡然，史进进，刘军杰，张振中，李景虹；一种线粒体DNA突变诱导释放药物的复合物，其制备方法及用途，2022.11.15，中国专利，申请，CN202211432387.9

3. 张开翔，李亚楠，黄迪，史进进，刘军杰，张振中，李景虹；一种一体化CRISPR-DNA 纳米器件及线粒体 DNA 单碱基突变的活体成像方法， 2022.8.30，中国专利，申请，CN202211050988.3

4. 张开翔，王丹钰，刘静文，高华，史进进，刘军杰，张振中；一种光响应递送 PDL1 适配体的 DNA 纳米免疫治疗药物的制备方法及应用， 2022.07.06，中国专利，申请，CN202210789372.1

5. 张开翔，王丹钰，刘静文，高华，史进进，刘军杰，张振中；一种用于富集检测复发肿瘤细胞的 DNA 水凝胶的制备方法及其应用， 2022.11.04，中国专利，申请，CN202211372899.0

6. 张开翔，王丹钰，段捷，高华，史进进，刘军杰，张振中；一种pH驱动聚集死亡受体5联合酶级联的DNA纳米弹簧的制备方法及应用，2023.04.23，中国专利，申请，CN202310440653.0

7. 张开翔，梁燕，王金金，韩文帅，徐辰璐，张振中；一种基于仿生矿化的 mRNA 纳米颗粒、制备方法及其在蛋白质表达中的应用，2022.10.24，中国专利，申请，CN202211299159.9

第一作者和通讯作者论文：

2023年

1. Li, Y.; Wu, Y.; Xu, R.; Guo, J.; Quan, F.; Zhang, Y.; Huang, D.; Pei, Y.; Gao, H.; Liu, W.; Liu J.; Zhang, Z.*; Deng, R.*; Shi, J.*; Zhang, K*. In vivo imaging of mitochondrial DNA mutations using an integrated nano Cas12a sensor. Nat. Commun. 2023, 14, 7722. (中科院一区，影响因子16.6)

2. Wang, D.#; Liu, J.#; Duan, J.; Yi, H.; Liu, J.; Song, H.*; Zhang, Z.*; Shi, J.*; Zhang, K.*, Enrichment and sensing tumor cells by embedded immunomodulatory DNA hydrogel to inhibit postoperative tumor recurrence. Nat. Commun. 2023, 14, 4511. (中科院一区，影响因子16.6)

3. Li, Y.; Xu, R.; Wu, Y.; Guo, J.; Quan, F.; Pei, Y.; Huang, D.; Zhao, X.; Gao, H.; Liu, J.*; Zhang, Z.*; Shi, J.*; Zhang, K.* Genotype-specific precision tumor therapy using mitochondrial DNA mutation-induced drug release system. Sci. Adv. 2023, 9, eadi1965. (中科院一区，影响因子13.6)

4. Zhang, J.; Guan, M.; Lv, M.; Liu, Y.; Zhang, H.*; Zhang, Z.*; Zhang, K.* Localized Imaging of Programmed Death-Ligand 1 on Individual Tumor-Derived Extracellular Vesicles for Prediction of Immunotherapy Response. ACS Nano 2023. 17, 20120–20134 (中科院一区，影响因子17.1)

5. Wang, D.; Yi, H.; Geng, S.; Jiang, C.; Liu, J.; Duan, J.; Zhang, Z.; Shi, J.; Song, H.; Guo, Z.*; Zhang, K.*, Photoactivated DNA Nanodrugs Damage Mitochondria to Improve Gene Therapy for Reversing Chemoresistance. ACS Nano 2023. 17, 16923–16934 (中科院一区，影响因子17.1)

6. Liang, Y.; Zhang, J.; Xu, C.; Wang, J.; Han, W.; Yang, J.; Wu, S.; An, J.; Liu, J.; Zhang, Z.*; Shi, J.*; Zhang, K.*, Biomimetic Mineralized CRISPR/Cas RNA Nanoparticles for Efficient Tumor-Specific Multiplex Gene Editing. ACS Nano 2023. 2023, 17, 15025–15043 (中科院一区，影响因子17.1)

7. Liang, Y.#; Han, W.#; Xu, C.; Wang, J.; Zhang, J.; An, J.; Liu, W.; Liu, J.; Zhang, Z.*; Shi, J.*; Zhang, K.*, Cascade‐Responsive “Oxidative Stress Amplifiers” Simultaneously Destroy Lysosomes and Co‐Deliver CRISPR/Cas9 to Enhance Oxidative Damage in Tumor. Adv. Funct. Mater. 2023, 2301256. (中科院一区，影响因子19.0)

8. Liang, Y.#; Wang, J.#; Xu, C.; Han, W.; Wu, S.; Zhang, J.; Liu, J.; Zhang, Z.*; Shi, J.*; Zhang, K.*, Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano‐Regulator for Reversal of Liver Fibrosis. Adv. Sci. 2023, 2300127. (中科院一区，影响因子15.1)

9. Li, Y.; Huang, D.; Pei, Y.; Wu, Y.; Xu, R.; Quan, F.; Gao, H.; Zhang, J.*; Hou, H.*; Zhang, K.*; Li, J., CasSABER for Programmable In Situ Visualization of Low and Nonrepetitive Gene Loci. Anal. Chem. 2023, 95, 2992-3001. (中科院一区，影响因子7.4)

10. Zhang, J.; Guan, M.; Ma, C.; Liu, Y.; Lv, M.; Zhang, Z.; Gao, H.*; Zhang, K.*, Highly Effective Detection of Exosomal miRNAs in Plasma Using Liposome-Mediated Transfection CRISPR/Cas13a. ACS Sens. 2023, 8, 565-575.(中科院一区，影响因子8.9)

11. Wang, D.; Duan, J.; Liu, J.; Yi, H.; Zhang, Z.; Song, H.*; Li, Y.*; Zhang, K.*, Stimuli-Responsive Self-Degradable DNA Hydrogels: Design, Synthesis, and Applications. Adv. Healthcare Mater. 2023, 2203031. (中科院一区，影响因子10.0)

12. Yu, S.; Cao, S.; He, S.; Zhang, K.*, Locus-Specific Detection of DNA Methylation: The Advance, Challenge, and Perspective of CRISPR-Cas Assisted Biosensors. Small Methods. 2023, 7, 2201624. (中科院二区，影响因子12.4)

13. Gao, H.#; Li, Y.#; Li, Y.; Qu, K.*; Zhang, K.*; Li, J. Detection of antibiotic-resistance genes in bacterial pathogens using a Cas12a/3D DNAzyme colorimetric paper sensor. Fundamental Research 2023. DOI: 10.1016/j.fmre.2023.04.011. (国产期刊，影响因子6.2)

2022年

14. Zhang, T.#; Deng, R.*#; Wang, Y.#; Wu, C.#; Zhang, K.#; Wang, C.; Gong, N.; Ledesma-Amaro, R.; Teng, X.; Yang, C.; Xue, T.; Zhang, Y.; Hu, Y.; He, Q.; Li, W. *; Li, J*, A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution. Nat. Biomed. Eng. 2022, 6, 957-967. (中科院一区，影响因子28.1)

15. Shi, J.#; Nie, W.#; Zhao, X.; Yang, X.; Cheng, H.; Zhou, T.; Zhang, Y.*; Zhang, K.*; Liu, J.*, An Intracellular Self-Assembly-Driven Uninterrupted ROS Generator Augments 5-Aminolevulinic-Acid-Based Tumor Therapy. Adv. Mater. 2022, 34, 2201049. (中科院一区，影响因子29.4)

16. Zhang, K.#; Ma, Y.#; Wang, D.; Liu, J.; An, J.; Li, Y.; Ma, C.; Pei, Y.; Zhang, Z.*; Liu J.*; Shi, J.*, In Vivo Activation of T-Cell Proliferation by Regulating Cell Surface Receptor Clustering Using a pH-Driven Interlocked DNA Nano-Spring. Nano Lett. 2022, 22, 1937-1945. (中科院一区，影响因子10.8)

17. Shi, J.#; Yang, Y.#; Yin, N.; Liu, C.; Zhao, Y.; Cheng, H.; Zhou, T.; Zhang, Z.*; Zhang, K.*, Engineering CXCL12 Biomimetic Decoy‐Integrated Versatile Immunosuppressive Nanoparticle for Ischemic Stroke Therapy with Management of Overactivated Brain Immune Microenvironment. Small Methods. 2022, 6, e2101158. (中科院二区，影响因子12.4)

18. Liang, Y.; Wu, S.; Han, W.; Wang, J.; Xu, C.; Shi, J.; Zhang, Z.*; Gao, H.*; Zhang, K.*; Li, J., Visualizing Single-Nucleotide Variations in a Nuclear Genome Using Colocalization of Dual-Engineered CRISPR Probes. Anal. Chem. 2022, 94, 11745-11752. (中科院一区，影响因子7.4)

19. Li, Y.#; Pei, Y.#; Huang, D.; Xu, R.; Wu, Y.; Li, Y.; Zhou, R.; Wei, Y.; Tan, X.; Gao, H.; Shi, J.; Zhang, Z.*; Zhang, K.*; Li, J.*, Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes. Biosens. Bioelectron. 2022, 216, 114641. (中科院一区，影响因子12.6)

20. Yu, W.; Xuan, C.; Liu, B.; Zhou, L.; Yin, N.; Gong, E.; Zhang, Z.; Li, Y.*; Zhang, K.*; Shi, J.*, Carrier-free programmed spherical nucleic acid for effective ischemic stroke therapy via self-delivery antisense oligonucleotide. Nano Res. 2022, 16, 735-745. (中科院一区，影响因子9.9)

21. Wang, D.; Liu, J.; Duan, J.; Ma, Y.; Gao, H.; Zhang, Z.; Liu, J.*; Shi, J.*; Zhang, K.*, Photocontrolled Spatiotemporal Delivery of DNA Immunomodulators for Enhancing Membrane-Targeted Tumor Photodynamic Immunotherapy. ACS Appl. Mater. Interfaces. 2022, 14, 44183-44198. (中科院二区，影响因子9.5)

22. Yu, W.; Yin, N.; Yang, Y.; Xuan, C.; Liu, X.; Liu, W.; Zhang Z.; Zhang, K.*; Liu, J.*; Shi, J.*, Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection. Acta Biomater. 2022, 140, 625-640. (中科院一区，影响因子9.7)

2021年

23. Shi, J.#; Wang, D.#; Ma, Y.; Liu, J.; Li, Y.; Reza, R.; Zhang, Z.; Liu, J.*; Zhang, K.*, Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. Small. 2021, 17, e2104722. (中科院一区，影响因子15.1)

24. Liu, J.#; Yu, W.#; Han, M.; Liu, W.; Zhang, Z.*; Zhang, K.*; Shi, J.*, A specific “switch-on” type magnetic resonance nanoprobe with distance-dominate property for high-resolution imaging of tumors. Chem. Eng. J. 2021, 404, 126496. (中科院一区，影响因子16.7)

25. Liu, J.#; Jin, Y.#; Song, Z.; Xu, L.; Yang, Y.; Zhao, X.; Wang, B.; Liu, W.; Zhang, K.*; Zhang, Z.*; Shi, J.*, Boosting tumor treatment by dredging the hurdles of chemodynamic therapy synergistic ion therapy. Chem. Eng. J. 2021, 411. (中科院一区，影响因子16.7)

26. Li, A.#; Liang, C.#; Xu, L.; Wang, Y.; Liu, W.; Zhang, K.*; Liu, J.*; Shi, J.*, Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation. Acta Pharm. Sin. B 2021, 11, 1329-1340. (中科院一区，影响因子14.9)

27. Liu, J.#; Zhao, X.#; Nie, W.; Yang, Y.; Wu, C.; Liu, W.; Zhang, K.*; Zhang, Z.*; Shi, J.*, Tumor cell-activated “Sustainable ROS Generator” with homogeneous intratumoral distribution property for improved anti-tumor therapy. Theranostics 2021, 11, 379-396. (中科院一区，影响因子11.6)

28. Guo, Y.*; Wu, D.; Zhang, X.; Zhang, K.*; Luo, Y.*, Biomolecules in cell-derived extracellular vesicle chariots as warriors to repair damaged tissues. Nanoscale 2021, 13, 16017-16033. (中科院一区，影响因子8.3)

2020年

29. Zhang, K.; Deng, R.; Gao, H.; Teng, X.; Li, J.*, Lighting up single-nucleotide variation in situ in single cells and tissues. Chem. Soc. Rev. 2020, 49, 1932-1954. (中科院一区，影响因子54.6)

30. Zhang, J.#; Shi, J.#; Zhang, H.; Zhu, Y.; Liu, W.; Zhang, K.*; Zhang, Z.*, Localized fluorescent imaging of multiple proteins on individual extracellular vesicles using rolling circle amplification for cancer diagnosis. J. Extracell. Vesicles 2020, 10, e12025. (中科院一区，影响因子25.8)

31. Zhang, K.#; Qin, S.#; Wu, S.; Liang, Y.; Li, J.*, Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level. Chem. Sci. 2020, 11, 6352-6361. (中科院一区，影响因子9.8)

32. Zhang, K.#; Li, Y.#; Liu, J.; Yang, X.; Xu, Y.; Shi, J.*; Liu, W.*; Li, J.*, Y-Shaped Circular Aptamer–DNAzyme Conjugates for Highly Efficient in Vivo Gene Silencing. CCS Chemistry 2020, 2, 631-641. (影响因子11.2)

33. Shi, J.#; Yang, X.#; Li, Y.; Wang, D.; Liu, W.; Zhang, Z.; Liu, J.*; Zhang, K.*, MicroRNA-responsive release of Cas9/sgRNA from DNA nanoflower for cytosolic protein delivery and enhanced genome editing. Biomaterials 2020, 256, 120221. (中科院一区，影响因子12.5)

34. Shi, J.#; Yu, W.#; Xu, L.; Yin, N.; Liu, W.; Zhang, K.*; Liu, J.*; Zhang, Z.*, Bioinspired Nanosponge for Salvaging Ischemic Stroke via Free Radical Scavenging and Self-Adapted Oxygen Regulating. Nano Lett. 2020, 20, 780-789. (中科院一区，影响因子11.2)

35. Liu, J.#; Zhu, C.#; Xu, L.; Wang, D.; Liu, W.; Zhang, K.*; Zhang, Z.*; Shi, J.*, Nanoenabled Intracellular Calcium Bursting for Safe and Efficient Reversal of Drug Resistance in Tumor Cells. Nano Lett. 2020, 20, 8102-8111. (中科院一区，影响因子11.2)

36. Liu, J.; Liu, W.; Zhang, K.*; Shi, J.*; Zhang, Z.* A Magnetic Drug Delivery System with "OFF-ON" State via Specific Molecular Recognition and Conformational Changes for Precise Tumor Therapy. Adv. Healthcare Mater. 2020, e1901316. (中科院一区，影响因子9.9)

37. Yan, Y.#; Zhang, K.#; Wang, H.; Liu, W.; Zhang, Z.; Liu, J.*; Shi, J.*, A fullerene based hybrid nanoparticle facilitates enhanced photodynamic therapy via changing light source and oxygen consumption. Colloids Surface B. 2020, 186, 110700. (中科院二区，影响因子5.3)

38. Zhang, K.; Liu, J.; Song, Q.; Wang, D.; Shi, J.*; Zhang, H.*; Li, J.*, Multifunctional DNA Nanoflowers for Autophagy Inhibition and Enhanced Antitumor Chemotherapy. Chem. J. Chinese U. 2020, 41, 1461-1469. (国产期刊，中科院四区，影响因子0.65)

2019年

39. Zhang, K.; Gao, H.; Deng, R.; Li, J.* Emerging Applications of Nanotechnology for Controlling Cell-Surface Receptor Clustering. Angew. Chem. Int. Ed. 2019, 58, 4790-4799. (中科院一区，影响因子12.9)

40. Zhang, K.#; Liu, J.#; Song, Q.; Yang, X.; Wang, D.; Liu, W.; Shi, J.*; Zhang, Z.* DNA Nanosponge for Adsorption and Clearance of Intracellular miR-21 and Enhanced Antitumor Chemotherapy. ACS Appl. Mater. Interfaces. 2019, 11, 46604-46613. (中科院二区，影响因子8.7)

41. Gao, H.; Zhang, K.*; Teng, X.; Li, J.* Rolling circle amplification for single cell analysis and in situ sequencing. TrAC, Trends Anal. Chem. 2019, 121, 115700. (中科院一区，影响因子9.8)

42. Zhang, K.#; Yue, Y.#; Wu, S.; Liu, W.*; Shi, J.*; Zhang, Z.* Rapid Capture and Non-destructive Release of Extracellular Vesicles using Aptamer-based Magnetic Isolation. ACS Sens. 2019, 4, 1245-1251. (中科院一区，影响因子7.3)

43. Liu, J.#; Zhang, Y.#; Liu, W.; Zhang, K.*; Shi, J.*; Zhang, Z.* Tumor Antigen Mediated Conformational Changes of Nanoplatform for Activated Photodynamic Therapy. Adv. Healthcare Mater. 2019, e1900791. (中科院一区，影响因子7.3)

44. Li, Y.; Zhai, Y.; Liu, W.; Zhang, K.*; Liu, J.*; Shi, J.*; Zhang, Z., Ultrasmall nanostructured drug based pH-sensitive liposome for effective treatment of drug-resistant tumor. J Nanobiotechnol. 2019, 17, 117. (中科院一区，影响因子6.5)

45. Liu, W.#; Zhang, K.#; Zhuang, L.; Liu, J.; Zeng, W.; Shi, J.*; Zhang, Z.*, Aptamer/photosensitizer hybridized mesoporous MnO(2) based tumor cell activated ROS regulator for precise photodynamic therapy of breast cancer. Colloids Surf. B. Biointerfaces 2019, 184, 110536. (中科院二区，影响因子4.4)

2015-2018年

46. Zhang, K.; Deng, R.; Teng, X.; Li, Y.; Sun, Y.; Ren, X.; Li, J.* Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR/Cas9-Mediated Proximity Ligation Assay. J. Am. Chem. Soc. 2018, 140, 11293-11301. (中科院一区，影响因子14.7)

47. Zhang, K.; Deng, R.; Sun, Y.; Zhang, L.; Li, J.* Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands. Chem. Sci. 2017, 8, 7098-7105. (中科院一区，影响因子9.1)

48. Zhang, K.; Deng, R.; Li, Y.; Zhang, L.; Li, J.* Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification. Chem. Sci. 2016, 7, 4951-4957. (中科院一区，影响因子8.7)

49. Zhang, K.; Kang, D. K.; Ali, M. M.; Liu, L.; Labanieh, L.; Lu, M.; Riazifar, H.; Nguyen, T. N.; Zell, J. A.; Digman, M. A.; Gratton, E.; Li, J.*; Zhao, W.* Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection. Lab Chip. 2015, 15, 4217-4226. (中科院一区，影响因子5.6)