主持国家自然科学基金重大项目子课题1项、国家重点研发计划项目子课题1项（经费169万元）；主持和参与其他国家及省部级项目10余项。获河南省科学技术进步奖一等奖1项、二等奖1项；河南省教育厅科技成果一等奖3项；获授权发明专利28件，获软件著作权4件；发表研究论文50余篇。 主持项目： 1. 国家自然科学基金重大项目子课题主持,潜在流行风险动物源性人兽共患病毒种间传播的干预阻断技术（32192454）, 2022-01-01至2026-12-31,185.9万元。 2. 国家重点研发计划项目子课题主持, 2019YFC1604501,抗生素及抗性基因生物毒素在食品原料中的迁移转化与富集机制, 2019-12至2022-12, 169万元。 3. 郑州大学青年人才企业合作创新团队支持计划项目，莫西沙星免疫层析快速检测技术研究，2021/06-2022/06，10万元，主持。 4. 国家卫生健康委出生缺陷预防重点实验室开放基金，人巨细胞病毒（HCMV）快速检测技术在出生缺陷预防中的应用研究，2021/06-2022/06，9万元，主持。 代表性论文 1.Chen Y,Zhang S, Zhang G, et al. Screening and identification of B cell epitope within the major capsid protein L1 of HPV 52, using monoclonal antibodies[J]. Journal of Virological Methods, 2023: 114855. 2.Chen Y, Liu Y, Wang A, et al.Human papillomavirus L1 protein expressed inEscherichia coliself-assembles into virus-like particles that are highly immunogenic. Virus Research, 2016, (220):97–103. 3.Chen Y, Liu Y, Zhang G, et al.Cloning and characterization of the IgA Fc receptor from swine [J]. J. Microbiol. Biotechnol, 2016, 26(0), 1–7. 4. Wang A, Wang H,Chen Y,et al. A Duplex TaqMan probe-based real-time PCR Method for Rapid Detection and Differentiation of the Classical and VB Strains of Human Immunodeficiency Virus. Science China-Life Sciences, 2023, 59: 1-4. 5. Wang A, Zhou Y,Chen Y,et al. Electrochemical immunosensor for ultrasensitive detection of human papillomaviruse type 16 L1 protein based on Ag@ AuNPs-GO/SPA[J]. Analytical Biochemistry, 2023, 660: 114953. 6. Li G, Wang A,Chen Y,et al. Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein[J]. Frontiers in Immunology, 2021, 12:635677. 7. Li N,Zhang G,Chen Y., et al. Identification of the Mimotopes within the major capsid protein L1 of human papillomavirus types 18 and 45, using neutralizing monoclonal antibodies[J]. International Journal of Biological Macromolecules, 2021, 174(31)：587-595. 8. Liu Y , Wang J ,Chen Y, et al. Identification of a dominant linear epitope on the VP2 capsid protein of porcine parvovirus and characterization of two monoclonal antibodies with neutralizing abilities[J]. International Journal of Biological Macromolecules, 2020, 163: 2013-2022 9. Wang J , Liu Y ,Chen Y, et al. Large-scale manufacture of VP2 VLP vaccine against porcine parvovirus in Escherichia coli with high-density fermentation[J]. Applied Microbiology and Biotechnology, 2020, 104(26)：3847-3857. 10. Zhang T，Liu Y，ChenY，et al. A single dose glycoprotein D-based subunit vaccine against pseudorabies virus infection[J]. Vaccine, 2020, 38( 39):6153-6161. 授权发明专利 1. 一种BTV1 VP2的原核表达制备方法ZL201910618587.5 2. 抗ASFV p54蛋白单克隆抗体的制备方法及其应用ZL202011607066.9 3. 编码可溶性HPV23 L1蛋白的基因及其重组质粒的构建与应用ZL202110615459.2 4. 抗HPV16 L1蛋白单克隆抗体及其制备方法和应用ZL201810332746.0 5. 表达可溶性人乳头瘤病毒16亚型L1蛋白的重组质粒及其表达方法ZL201510887156.0 6. 抗PCV2单克隆抗体及其制备方法和应用ZL201810332092.1 7. 编码可溶性HPV58L1蛋白的基因及其重组质粒的构建与应用ZL202110632917.3 8. 一种猪瘟病毒和猪伪狂犬病毒的二联金标检测试纸及其制备方法ZL 201811091645.5 9. 抗HPV39 L1蛋白单克隆抗体及其制备及应用ZL 202111336152.5 10. 抗非洲猪瘟病毒CD2v蛋白单克隆抗体、制备方法及应用ZL202110919068.X |
