个人简介
姓名：吕永坤
学历：博士研究生
学位：工学博士
招生专业：生物化工、制药工程、材料与化工等
工作单位：郑州大学 化工学院
职称：副教授
邮箱：yklv@zzu.edu.cn
主要研究方向
微生物合成生物学和代谢工程，微生物细胞工厂，发酵工程。
工作经历
20220.01 – 今 郑州大学 化工学院 直聘副教授、副教授
2019.06 – 2019.12 郑州大学 化工学院 讲师
教育经历
2017.10 - 2018.10 美国马里兰大学巴尔的摩郡分校(UMBC) 博士生联合培养
2014.09 – 2019.01 江南大学 发酵工程 博士研究生
2011.09 - 2013.12 江南大学 轻工技术与工程 硕士研究生
2007.09 - 2011.06 河南农业大学 生物科学和经济学（双学位） 本科
科研项目
1. 国家自然科学基金青年项目，2023.01 – 2025.12，主持，结题；
2. 中国博士后科学基金面上项目（76批），2025.01 – 2026.12，主持，在研；
3. 河南省博士后科学基金，2024.01 – 2025.12，主持，结题；
4. 河南省青年人才托举工程项目，2021.01 – 2022.12，主持，结题；
5. 河南省重点研发与推广专项（科技攻关）项目，2020.01 – 2021.12，主持，结题；
6. 中国博士后科学基金面上项目（66批），2020.01 – 2021.12，主持，结题；
7. 郑州大学青年教师启动基金（拔尖），2020.01-2022.12，主持，结题。
学术概况
长期从事微生物合成生物学和代谢工程研究。解析了药用植物天然产物（异）水飞蓟宾的原生合成途径和转运机制（Lv Y. et al., Plant J., 2017）；设计构建并优化了微生物细胞反应器，实现了水飞蓟宾及其前体的高效生物合成（Lv Y. et al., Green Chem., 2019）。利用解脂耶氏酵母基因组26s rDNA特征，结合Cre-loxP系统，开发出了解脂耶氏酵母基因组随机迭代整合工具包（Lv Y. et al., ACS Synth. Biol., 2019）。将工具包在多种黄酮类植物天然产物从头合成细胞工厂中进行了验证和应用（Lv Y. et al., ACS Synth. Biol., 2019）。设计构建了双层动态调控系统，实现了目标产物合成和细胞生长代谢流的再分配和动态平衡，以及细胞工厂长期发酵的稳定性（Lv Y. et al., Metab. Eng., 2020）。设计、验证了反式-草蒿脑和反式-茴香脑从头合成细胞工厂，实现了在微生物底盘中的从头合成（Lv Y. et al., ACS Sustainable Chem. Eng., 2026.）。验证了1种戊糖Izumoring途径，实现了转化木糖高效合成D-阿洛酮糖（Xu J. et al., J. Agric. Food Chem. 2024）；设计了基于人工基因线路的D-阿洛酮糖合成途径体内连续定向进化策略（Lv Y. et al., J. Agric. Food Chem. 2025）。以第一/通讯（含共同）作者在领域内主流期刊发表论文20篇，已获授权发明专利12项（含美国专利2项）。获江南大学优秀博士学位论文，入选河南省科协青年人才托举工程。担任Systems Microbiology and Biomanufacturing期刊青年编委；Biotechnology Advances、Journal of Agricultural and Food Chemistry、ACS Sustainable Chemistry & Engineering、Biotechnology Journal、Chemical Engineering Journal、Chem & Bio Engineering和Bioresource Technology等期刊审稿人。
一作/通讯论文 (*表示通讯作者)：
(1) Yongkun Lv, Shuai Ding, Jinmian Chang*, Zhouyan Guo, Ying Xiao, Shiming Gu, Anqi Zhao, Shilei Wang, Weigao Wang, Yameng Xu, Jingliang Xu*, and Peng Xu*. Demonstrating and engineering the de novo synthesis of two aromatic chemicals estragole and anethole in Escherichia coli. ACS Sustainable Chem. Eng., 2026. (Accepted, 中科院1区TOP)
(2) Yongkun Lv, Song Chen, Lijuan Zhu, Zhouyan Guo, Zhenhua Chen, Anqi Zhao, Shilei Wang, Mengzhuo Shi, Weigao Wang, Yameng Xu, Peng Xu*, and Jingliang Xu*. Improving D-allulose production via alditol oxidase directed evolution assisted by an artificial genetic circuit. J. Agric. Food Chem. 2025, 73(22): 13676–13683. (中科院1区TOP)
(3) Jingliang Xu, Hanyu Dong, Song Chen, Jinmian Chang, Weiping Zhang, Anqi Zhao, Md. Asraful Alam, Shilei Wang, Weigao Wang, Jianguo Zhang, Yongkun Lv*, and Peng Xu. Producing D-Ribose from D-Xylose by Demonstrating a Pentose Izumoring Route. J. Agric. Food Chem. 2024, 72(49): 27304–27313. (中科院1区TOP)
(4) Yongkun Lv, Jinmian Chang, Weiping Zhang, Hanyu Dong, Song Chen, Xian Wang, Anqi Zhao, Shen Zhang, Md Asraful Alam, Shilei Wang, Chaojun Du, Jingliang Xu*, Weigao Wang*, and Peng Xu*. Improving Microbial Cell Factory Performance by Engineering SAM Availability. J. Agric. Food Chem. 2024, 72(8): 3846-3871. (中科院1区TOP)
(5) Yongkun Lv and Weigao Wang. Metabolic design–build–test–learn cycle used for the biosynthesis of plant-derived bioactive compounds. Yongjun Wei, Xiao-jun Ji, and Mingfeng Cao (Ed.), Engineering Biology for Microbial Biosynthesis of Plant-Derived Bioactive Compounds. Academic Press. 2024, 105-120. Doi: https://doi.org/10.1016/B978-0-443-15558-1.00006-0. (Book chapter)
(6) Hui Zhang, Anqi Zhao, Lingbo Qu, Wenlong Xiong, Md. Asraful Alam, Jixing Miao, Weigao Wang, Jingliang Xu*, and Yongkun Lv*. Engineering an efficient whole-cell catalyst for D-allulose production from glycerol. Biotechnol. J. 2023, 18(7): e2200600. (Top Cited Article)
(7) Qing Jia, Hui Zhang, Anqi Zhao, Lingbo Qu, Wenlong Xiong, Md. Asraful Alam, Jixing Miao, Weigao Wang, Feihu Li*, Jingliang Xu*, and Yongkun Lv*. Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis. Front. Bioeng. Biotech. 2023, 11.
(8) Zhen Fang*, Narenkumar Jayaraman, Yongkun Lv*, and Zhengshan Luo*. Editorial: Cofactor regeneration technologies for microbial cell factory. Front. Microbiol. 2023, 14:1196002.
(9) Lijuan Zhu#, Hui Zhang#, Shiyuan Wang, Anqi Zhao, Lingbo Qu, Wenlong Xiong, Md. Asraful Alam, Wenlong Ma, Yongkun Lv*, and Jingliang Xu*, Screening a Panel of Acid-producing Strains by Developing a High-throughput Method. Biotechnol. Bioprocess Eng. 2022, 27 (5), 784-791.
(10) Abdul Rahman Abbasi, Jinle Liu, Zhi Wang, Anqi Zhao, Hanjie Ying, Lingbo Qu, Md. Asraful Alam, Wenlong Xiong, Jingliang Xu*, and Yongkun Lv*. Recent advances in producing sugar alcohols and functional sugars by engineering Yarrowia lipolytica, Front. Bioeng. Biotech. 2021, 9: 648382.
(11) Yongkun Lv, Yang Gu, Jingliang Xu*, Jingwen Zhou*, and Peng Xu*. Coupling metabolic addiction with negative autoregulation to improve strain stability and pathway yield, Metabolic Engineering, 2020, 61: 79-88. (中科院1区TOP，ESI高被引论文)
(12) Yongkun Lv, Shuai Qian, Guocheng Du, Jian Chen, Jingwen Zhou, and Peng Xu*. Coupling feedback genetic circuits with growth phenotype for dynamic population control and intelligent bioproduction, Metabolic Engineering, 2019, 54: 109-116. (中科院1区TOP)
(13) Yongkun Lv, Sha Xu, Yunbin Lyu, Shenghu Zhou, Guocheng Du, Jian Chen, and Jingwen Zhou*. Engineering enzymatic cascades for the efficient biotransformation of eugenol and taxifolin to silybin and isosilybin, Green Chemistry, 2019, 21(7): 1660-1667. (中科院1区TOP，Back Cover论文)
(14) Yongkun Lv, Harley Edwards, Jingwen Zhou, and Peng Xu*. Combining 26s rDNA and the Cre-loxP System for Iterative Gene Integration and Efficient Marker Curation in Yarrowia lipolytica, ACS Synthetic Biology, 2019, 8(3): 568-576.
(15) Yongkun Lv, Monireh Marsafari, Mattheos Koffas, Jingwen Zhou, and Peng Xu*. Optimizing Oleaginous Yeast Cell Factories for Flavonoids and Hydroxylated Flavonoids Biosynthesis, ACS Synthetic Biology, 2019, 8(11): 2514-2523.
(16) Yongkun Lv, Xiaozhong Cheng, Di Wu, Guocheng Du, Jingwen Zhou*, and Jian Chen*. Improving bioconversion of eugenol to coniferyl alcohol by in situ eliminating harmful H2O2. Bioresource Technology. 2018, 267:578-83. (中科院1区TOP)
(17) Yongkun Lv, Song Gao, Sha Xu, Guocheng Du, Jingwen Zhou*, and Jian Chen*. Spatial organization of silybin biosynthesis in milk thistle [Silybum marianum(L.) Gaertn]. The Plant Journal. 2017, 92(6):995-1004. (中科院1区TOP)
(18) Yongkun Lv, Xiaozhong Cheng, Guocheng Du, Jingwen Zhou*, and Jian Chen*. Engineering of an H2O2 auto-scavenging in vivo cascade for pinoresinol production. Biotechnology and Bioengineering. 2017, 114(9):2066-74. (Spotlight论文)
(19) 吕永坤;堵国成;陈坚;周景文*; 合成生物学技术研究进展; 生物技术通报; 2015, 31(4): 134-148.
(20) 吕永坤;周景文;刘龙*; 氮代谢调控相关蛋白质泛素化修饰筛选; 食品与生物技术学报 . 2014, 33(6): 576-582.
(21) Yongkun Lv, Xinrui Zhao, Long Liu, Guocheng Du, Jingwen Zhou*, and Jian Chen*, A simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae: Gap1p as an example. Journal of Microbiological Methods. 2013, 94 (1): 25-29.
毕业生：
2025届硕士：
陈松：江南大学读博，获郑州大学优秀学位论文
陈振华：智享研发工程师
李宇航（协助培养）：今世缘酒业
2024届硕士：
常金勉：牧原实验室研发工程师，获郑州大学优秀学位论文
董寒玉（协助培养）：牧原实验室研发工程师
